The Gadsden flag, featuring a rattlesnake and the word "Don't Tread on Me

https://deanmwsm217.wordcanopy.com/posts/reenacting-the-past-the-significance-of-using-authentic-civil-war-flags-2

The Gadsden flag, providing a rattlesnake and the word "Don't Tread on Me," become adopted by a few Confederate sets as a symbol of resistance opposed to perceived oppression.